Difference between revisions of "Part:BBa J45100:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | BSMT CDS taken from GenBank. The following primers were used for cloning: | |
Designed Forward Strand (1 and 2): | Designed Forward Strand (1 and 2): | ||
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===Source=== | ===Source=== | ||
| − | Species: Petunia ''X. hybrida'' from GenBank (AY233465); (also available: ''A. thaliana'' and ''N. suaveolens'') | + | Species: Petunia ''X. hybrida'' from GenBank (AY233465); (also available: ''A. thaliana'' and ''N. suaveolens''). We obtained our version of BSMT from N. Dudareva. |
===References=== | ===References=== | ||
Latest revision as of 04:31, 31 October 2006
Wintergreen odor enzyme (BSMT1) generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 746
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 287
Design Notes
BSMT CDS taken from GenBank. The following primers were used for cloning:
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
Source
Species: Petunia X. hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens). We obtained our version of BSMT from N. Dudareva.
References
<biblio>
- Underwood05 pmid=15849311
- Negre03 pmid=14630969
</biblio>