Difference between revisions of "Part:BBa K1413041:Experience"
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===Applications of BBa_K1413041===
 
===Applications of BBa_K1413041===
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We used OriVR6Kgamma in the pNK2 (BBa_K141344). This plasmid contains a particular origin of replication OriVR6Kgamma. This oriVR6K gamma is controlled by the pi protein, which is encoded by the pir gene. Indeed, the pi protein allows the replication of the plasmid by binding a particular site in the ORI sequence. Hence, the oriVR6K gamma can only be replicated in a bacterial strain producing the pi protein. Also we used the cells DH5alpha pri, which replicated this plasmid. With other bacteria like BL21, or DH5alpha there wasn't replication.  
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We used OriVR6Kgamma in <a href="https://parts.igem.org/Part:BBa_K1413044">BBa_K1413044</a> taken from pNK2.
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===User Reviews===
 
===User Reviews===

Latest revision as of 18:26, 1 November 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1413041


We used OriVR6Kgamma in BBa_K1413044 taken from pNK2.

User Reviews

UNIQc6e4d411d5a7ebc4-partinfo-00000001-QINU UNIQc6e4d411d5a7ebc4-partinfo-00000002-QINU