Difference between revisions of "Part:BBa K1403002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Codon | + | Codon optimized using [http://eu.idtdna.com/CodonOpt IDT online tool]. We checked that there were no restriction sites for EcoRI, SpeI, XbaI and PstI using the software Geneious. |
− | + | ||
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===Source=== | ===Source=== | ||
− | + | AgaA sequence ([http://www.ncbi.nlm.nih.gov/nuccore/AF534871.1 Genbank: AF534871.1]) codon optimized for <i>E. coli 12</i> (IDT online tool) | |
===References=== | ===References=== |
Latest revision as of 14:24, 28 October 2014
AgaA expression (3M2H-> 3H3MH)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 288
Illegal BamHI site found at 519 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 181
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized using [http://eu.idtdna.com/CodonOpt IDT online tool]. We checked that there were no restriction sites for EcoRI, SpeI, XbaI and PstI using the software Geneious.
Source
AgaA sequence ([http://www.ncbi.nlm.nih.gov/nuccore/AF534871.1 Genbank: AF534871.1]) codon optimized for E. coli 12 (IDT online tool)
References
- [http://ncbi.nlm.nih.gov/pubmed/12468539 "A specific bacterial aminoacylase cleaves odorant precursors secreted in the human axilla." Acuna G. et al ,2003]