Difference between revisions of "Part:BBa J45100:Design"

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<partinfo>BBa_J45100 short</partinfo>
 
<partinfo>BBa_J45100 short</partinfo>
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===Design Notes===
 
===Design Notes===
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See <bbpart>J45099</bbpart>
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BSMT CDS taken from GenBank.  The following primers were used for cloning:
 +
 
 +
Designed Forward Strand (1 and 2):
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5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G <b>ATG GAA GTT GTT GAA GTT C</b> -3'<br>
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*LENGTH: 19 <br>
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*GC CONTENT: 36.8 % <br>
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*MELT TEMP: 47.8 ºC <br>
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<br>
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Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TA<b>T TAA TTT ATT TTG GTC AAG GAG</b> -3'<br>
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*LENGTH: 22 <Br>
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*GC CONTENT: 27.3 % <br>
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*MELT TEMP: 46.6 ºC <br>
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<br>
  
 
===Source===
 
===Source===
  
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This is made from a biobrick promoter and our biobrick wintergreen generating device.  
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Species: Petunia ''X. hybrida'' from GenBank (AY233465); (also available: ''A. thaliana'' and ''N. suaveolens'').  We obtained our version of BSMT from N. Dudareva.
  
 
===References===
 
===References===
 +
<biblio>
 +
#Underwood05 pmid=15849311
 +
#Negre03 pmid=14630969
 +
</biblio>

Latest revision as of 04:31, 31 October 2006

Wintergreen odor enzyme (BSMT1) generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 746
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 287


Design Notes

BSMT CDS taken from GenBank. The following primers were used for cloning:

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG GAA GTT GTT GAA GTT C -3'

  • LENGTH: 19
  • GC CONTENT: 36.8 %
  • MELT TEMP: 47.8 ºC


Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

  • LENGTH: 22
  • GC CONTENT: 27.3 %
  • MELT TEMP: 46.6 ºC


Source

Species: Petunia X. hybrida from GenBank (AY233465); (also available: A. thaliana and N. suaveolens). We obtained our version of BSMT from N. Dudareva.

References

<biblio>

  1. Underwood05 pmid=15849311
  2. Negre03 pmid=14630969

</biblio>