Difference between revisions of "Part:BBa K1333111"
(→Usage and Biology) |
|||
Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. | DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication. | ||
+ | |||
+ | [[Image:Sysu SOS bypass.jpg|thumb|center|400px|SOS error prone bypass]] | ||
===For more information please visit our wiki=== | ===For more information please visit our wiki=== |
Latest revision as of 01:58, 28 October 2014
DNAQ CDS
a subunit of DNA polymeraseIII
Usage and Biology
DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.
For more information please visit our wiki
http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutagenesis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 46
Illegal AgeI site found at 535 - 1000COMPATIBLE WITH RFC[1000]