Difference between revisions of "Part:BBa J36848:Design"
(→Design Notes) |
|||
| (One intermediate revision by the same user not shown) | |||
| Line 9: | Line 9: | ||
There is no spacer nucleotide between the end of the device and the SpeI restriction site. | There is no spacer nucleotide between the end of the device and the SpeI restriction site. | ||
| − | Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and | + | Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 00:23, 31 October 2006
Lac-inducible generator of Lpp-OmpA(46-66)-Streptavidin wild-type + His6tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 432
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 474
Illegal AgeI site found at 525 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
There is no spacer nucleotide between the end of the device and the SpeI restriction site.
Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. This does not apply between R0010 and B0034 where there are still two spacer nucleotides, and after B0034 where there is still one spacer nucleotide, because R0010 and B0034 are standard bioBrick parts.
Source
Assembly of BBa_J04500 and BBa_J36844