Difference between revisions of "Part:BBa K1333101"

Latest revision as of 16:22, 27 October 2014

RBS DNAQ

DNAQ is the proofreading subunit of E.coli DNA polymerase III.

Usage and Biology

DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.

For more information please visit our wiki

http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutation

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 192
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 64
Illegal AgeI site found at 553
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
 DNA polymerase III is the main enzyme during DNA replication. DNAQ encodes the epsilon subunit of DNA polymerase III which is responsible for the proofreading function. DNAQ has the 3’-5’ exonuclease activity. During the process of DNA replication ,as soon as base mispairing occur, DNAQ will cut off the newly synthesized base, which enable the right base to substitute its position. This guarantee the accuracy of DNA replication.
+===For more information please visit our wiki===
+http://2014.igem.org/Team:SYSU-China/content.html#Project/Result/Mutation
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