Difference between revisions of "Part:BBa K1499401:Experience"
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− | + | To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity. | |
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− | + | [[File:SBSiGEM2014_Wasp_Protein_Gel_Chitin.png|500px|thumb|center|<b>Figure 1.</b> C1 (BBa_K1499400) and C2 (BBa_K1499401) after chitin magnetic bead purification. C2 was successfully purified with chitin magnetic beads, suggesting that this protein has functional chitin-binding domains as predicted by PSI-BLAST.]] | |
===Applications of BBa_K1499401=== | ===Applications of BBa_K1499401=== | ||
+ | The protein encoded by this gene has shown potential chitin-binding activity. Pending assays for cellulose-binding activity and hydrophobicity, this protein could be used to waterproof chitin- and cellulose-based biomaterials – specifically in our case, fungal mycelium and bacterial cellulose. | ||
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 00:59, 25 October 2014
To purify this protein from cell lysate, we used the New England Biolabs Chitin Magnetic Beads protein purification kit, which isolates proteins that bind to chitin-coated magnetic beads. The following gel image shows that BBa_K1499401 (nicknamed C2) was successfully purified with the chitin magnetic beads, suggesting that it exhibits chitin-binding activity.
Applications of BBa_K1499401
The protein encoded by this gene has shown potential chitin-binding activity. Pending assays for cellulose-binding activity and hydrophobicity, this protein could be used to waterproof chitin- and cellulose-based biomaterials – specifically in our case, fungal mycelium and bacterial cellulose.
User Reviews
UNIQaad78c26ba14c7f9-partinfo-00000000-QINU UNIQaad78c26ba14c7f9-partinfo-00000001-QINU