Difference between revisions of "Part:BBa J36846"
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This construct codes for a protein which displays streptavidin on the cell surface. | This construct codes for a protein which displays streptavidin on the cell surface. | ||
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| + | NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:48, 30 October 2006
Lpp-OmpA(46-159)-Streptavidin wild-type + His6
Assembled construct of signal peptide of lipoprotein, five transmembrane domains of outer membrane protein A, and streptavidin, wild-type with His6 tag at the end.
This construct codes for a protein which displays streptavidin on the cell surface.
NOTE ABOUT THE SEQUENCE: The mixed site between parts is 'only' six base pairs, ACTAGA. There is no spacer T or G nucleotide. These spacer nucleotides have been placed in the results for "get selected sequence" as an automatic composite-parts addition for the BioBricks mixed site between assembled parts.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 485
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 527
Illegal AgeI site found at 578 - 1000COMPATIBLE WITH RFC[1000]