Difference between revisions of "Part:BBa K1456001"

Latest revision as of 22:07, 24 October 2014

Human Tissue Plasminogen Activator (htPA)

It has already been told that this protein is the most potent and efficient thrombolytic agent popular in clinical practice. It does this by activating plasminogen, a common anticoagulative factor zymogen found in normal bloodstream. By the conversion of plasminogen into plasmin, an enzyme that catalyzes the reaction of the cleavage of fibrin, the main clot component, thrombolysis begins to degrade the clot. (IUBMB Enzyme Nomeclature 1992; MetaCyc 2014)

This activity has been shown synthetically in E.coli and experimentally in vitro. (Pennica et al. 1983; Van de Werf et al. 1984)

According to the tPA assay results we obtained, we were able to prove that the tPA enzyme could be produced and secreted from the cell successfully hence also showing that tPA is functionally active.

Sequence and Features

Assembly Compatibility:

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 <p>This activity has been shown synthetically in E.coli and experimentally in vitro. (Pennica et al. 1983; Van de Werf et al. 1984)</p>
+[[File:ATOMS-314-TPA.jpg|center|]]
 </ul>
 <center>[[File:ATOMS-tpa results10.jpg|400px|]]</center><br/>
+*According to the tPA assay results we obtained, we were able to prove that the tPA enzyme could be produced and secreted from the cell successfully hence also showing that tPA is functionally active.
 <!-- Add more about the biology of this part here