Difference between revisions of "Part:BBa B0034:Design"

 
 
(2 intermediate revisions by the same user not shown)
Line 4: Line 4:
  
 
<partinfo>BBa_B0034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_B0034 SequenceAndFeatures</partinfo>
 +
  
 
===Design Notes===
 
===Design Notes===
 +
Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix. 
  
−
<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix.  <p>No secondary structures are formed in the given RBS region. Users should check      for secondary structures induced in the RBS by upstream and downstream elements      in the +50 to -50 region, as such structures will greatly affect the strength      of the RBS. <P>
+
No secondary structures are formed in the given RBS region. Users should check      for secondary structures induced in the RBS by upstream and downstream elements      in the +50 to -50 region, as such structures will greatly affect the strength      of the RBS.
  
 +
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
  
−
===Source===
 
  
−
--What is the source of this part?--
 
  
−
===References===
+
===Source===
  
  
−
===Files===
+
 
 +
===References===

Latest revision as of 01:21, 5 June 2006


RBS (Elowitz 1999) -- defines RBS efficiency


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix.

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>


Source

References