Difference between revisions of "Part:BBa J37015"

Latest revision as of 16:17, 20 August 2007

Prey Molecule Generator (AHL) + GFP Reporter

Prey Genotype + Reporter

Usage and Biology

Biology:

In nature, quorum sensing is used for cell-cell communication to co-ordinate particular behaviours or actions once the cell population has reached a sufficiently large size.
This part generates the quorum sensing molecule, AHL via a positive feedback loop:

The tetR promoter constitutively produces LuxR. AHL then forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI causes the production of AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.

The positive feedback loop is triggered once cell population reaches a certain size.
The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].

Usage:

This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule).

Other Uses:

This part could be used to amplify a signal: (see experience page)

A small amount of AHL present in the cell can be used to trigger the production of much more AHL via the positive feedback loop of this part.

This part could be used to monitor cell population size.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1724
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 2400

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-'''Usage:'''
-*This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule) using a positive feedback loop.
 '''Biology:'''
-*The positive feedback loop relies on leaky expression of LuxR by the tetR promoter which causes production of luxR. AHL already present in the cell forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI enzymatically produces AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
+*In nature, quorum sensing is used for cell-cell communication to co-ordinate particular behaviours or actions once the cell population has reached a sufficiently large size.
+*This part generates the quorum sensing molecule, AHL via a positive feedback loop:
+::*The tetR promoter constitutively produces LuxR. AHL then forms a complex with luxR and lux pR promoter, expressing LuxI and GFP. LuxI causes the production of AHL. GFP can be used to monitor transcription of LuxI as a check for the functionality of the part.
+*The positive feedback loop is triggered once cell population reaches a certain size.
 *The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Prey_Construct/Modelling transfer curve of AHL vs. Time].
+'''Usage:'''
+*This part was created by iGEM@Imperial 2006 to be used as the prey part in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to generate a the production of Acyl-Homoserine Lactone (a quorum sensing molecule).
+'''Other Uses:'''
+*This part could be used to amplify a signal: (see experience page)
+::*A small amount of AHL present in the cell can be used to trigger the production of much more AHL via the positive feedback loop of this part.
+*This part could be used to monitor cell population size.
 <span class='h3bb'>Sequence and Features</span>