Difference between revisions of "Part:BBa B0033:Design"

 
 
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<partinfo>BBa_B0033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_B0033 SequenceAndFeatures</partinfo>
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===Design Notes===
 
===Design Notes===
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Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix (&quot;RBS-3&quot; in figure 4-14 of thesis).   
  
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<P> <P>Varies from -6 to +1 region from original sequence to accomodate BioBricks      suffix ("RBS-3" in figure 4-14 of thesis).    <p>No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS. <P>
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No secondary structures are formed in the given RBS region. Users should      check for secondary structures induced in the RBS by upstream and downstream      elements in the +50 to -50 region, as such structures will greatly affect      the strength of the RBS.
  
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Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
  
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===Source===
 
  
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--What is the source of this part?--
 
  
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===References===
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===Source===
  
  
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===Files===
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===References===

Latest revision as of 01:21, 5 June 2006


RBS.4 (weaker) -- derivative of BBa_0030


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis).

No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.

Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>


Source

References