Difference between revisions of "Part:BBa K1321112:Design"

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===Source===
 
===Source===
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Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.
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PhytoC+CBD7  with LacI
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The LacI part came from BBa_J04500.
  
 
===References===
 
===References===

Latest revision as of 20:30, 21 October 2014


Phytochelatin (PC) EC20 fused to CBDcex with LacI promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. The LacI vector was fused to the protein fusion using the Xba and SpeI site.

Source

Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101. The LacI part came from BBa_J04500.

References