Difference between revisions of "Part:BBa K1321095:Design"
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===Design Notes=== | ===Design Notes=== | ||
Codon optimised for E.coli. | Codon optimised for E.coli. | ||
| − | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and | + | Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker. |
===Source=== | ===Source=== | ||
| − | + | Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101. | |
===References=== | ===References=== | ||
Latest revision as of 19:41, 21 October 2014
CBDcex fused to Phytochelatin (PC) EC20
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 333
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
Source
Phytochelatin was synthesized from Geneart and cloned into the psB1C3 backbone. CBDcex was requested from the registry, as it was submitted by the Freiburg team as BBa_K863101.