Difference between revisions of "Part:BBa K1391100:Design"

(→‎Design Notes)
(→‎Design Notes)
 
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This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts.
 
This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts.
 +
 +
This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
  
 
(Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)
 
(Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)

Latest revision as of 16:13, 30 October 2014


pEXPR_Gantenerumab Variable Human Ig-M Heavy Chain


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 306
    Illegal EcoRI site found at 480
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 306
    Illegal EcoRI site found at 480
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 306
    Illegal EcoRI site found at 480
    Illegal BglII site found at 1176
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 306
    Illegal EcoRI site found at 480
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 306
    Illegal EcoRI site found at 480
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is an IgM antibody with a variable region identical to the IgG antibody Gantenerumab which has shown therapeutic potential as an antibody against beta amyloid. This antibody is membrane bound and meant to be used in conjunction with the Gantenerumab Kappa Light Chain, CD79A, and CD79B to form a B-Cell Receptor Complex for extracellular sensing of beta amyloid. This particular chain has an IgM constant region fused to the Gantenerumab variable region and a leader sequence fused to the end of that for membrane localization.

This gene is expressed under a hEF1a promoter for constitutive high level expression in mammalian cells. It adheres to RFC 65 for mammalian parts.

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

(Ignore the RFC 65 link it is an automated link that links to a page unrelated to the iGEM RFC 65)

Source

Fusion protein of 2 human peptide sequences and 1 humanized murine sequence.

References