Difference between revisions of "Part:BBa K1448013"
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<partinfo>BBa_K1448013 short</partinfo> | <partinfo>BBa_K1448013 short</partinfo> | ||
| − | This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator | + | This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator [BBa_K1448007]. |
=== How to use === | === How to use === | ||
Latest revision as of 13:54, 19 October 2014
In E coli mono-terpene production cassette
This part contains the IPTG inducible tac promoter, RBS and erg20-2 generator [BBa_K1448007].
How to use
First, digest this part with SpeI.
Second, digest a BioBrickPart encoding a monoterpene synthase with SpeI and XbaI.
Third, mix and ligate both samples.
Various monoterpenes can be produced in E. coli cells containing this device and monoterpene synthase genes without exogenous GPP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]