Difference between revisions of "Part:BBa K1331002"
Danielle D (Talk | contribs) |
Danielle D (Talk | contribs) |
||
| Line 2: | Line 2: | ||
<partinfo>BBa_K1331002 short</partinfo> | <partinfo>BBa_K1331002 short</partinfo> | ||
| − | This part encodes a transcription regulatory protein | + | This part encodes a transcription regulatory protein rhlR. |
It includes the whole coding sequence and a part of downstream noncoding sequence of rhlR. | It includes the whole coding sequence and a part of downstream noncoding sequence of rhlR. | ||
Latest revision as of 13:54, 19 October 2014
RhlR regulatory protein from P. aeruginosa SQ6
This part encodes a transcription regulatory protein rhlR.
It includes the whole coding sequence and a part of downstream noncoding sequence of rhlR.
The purpose of remaining the noncoding sequence is for composition with our part BBa_K1331003. See our composite part rhlRI (BBa_K1331007).
Usage and Biology
RhlR is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase I.
It also functions as a transcriptional activator of elastase structural gene (lasB).
RhlR binds to autoinducer molecules C4-HSL which is synthesized by rhlI, and the complex positively regulates the promoter of rhlAB consequently, while rhlR alone negtively regulates the rhlAB promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 863
Illegal BamHI site found at 344 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 843
Illegal NgoMIV site found at 847 - 1000COMPATIBLE WITH RFC[1000]