Difference between revisions of "Part:BBa K1331007"

Latest revision as of 04:53, 19 October 2014

Modified rhlRI coding sequence from Pseudomonas aeruginosa SQ6

This part encodes both rhlR and rhlI. It is composited with BBa_K1331002 and BBa_K1331003.

Usage and Biology

The sequence we provide here includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.

The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 863
Illegal BamHI site found at 344
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 843
Illegal NgoMIV site found at 847
Illegal NgoMIV site found at 994
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1126

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1331007 short</partinfo>
-This part is combined with [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] and [https://parts.igem.org/Part:BBa_K1331003 BBa_K1331003].
+This part encodes both rhlR and rhlI. It is composited with [https://parts.igem.org/Part:BBa_K1331002 BBa_K1331002] and [https://parts.igem.org/Part:BBa_K1331003 BBa_K1331003].
-It includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.
+===Usage and Biology===
+The sequence we provide here includes both the coding sequence of rhlR, rhlI and the whole noncoding sequence between rhlR and rhlI.
+The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.
-The purpose of remaining the noncoding sequence is to perserve the promoter of rhlI in the part to ensure the normal expression of rhlI and synthesis of C4-HSL, which together with rhlR form a regulatory system of rhlAB transcription.
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