Difference between revisions of "Part:BBa K1331006"

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This part encodes both the subunit A and B of rhamnosyltransferase I. It is composited with [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] and [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004].  
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This is a composite part made of [https://parts.igem.org/Part:BBa_K1331001 BBa_K1331001] and [https://parts.igem.org/Part:BBa_K1331004 BBa_K1331004].  
  
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The part includes both the coding sequence of RhlA, RhlB and the whole noncoding sequence between RhlA and RhlB.
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===Usage and Biology===
  
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The purpose of remaining this noncoding sequence is to ensure the normal expression of rhamnosyltransferase I.
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This part encodes both the subunit A and B, and together make the functional rhamnosyltransferase I, the essential enzyme for rhamnolipid synthesis.
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This sequence includes both the coding sequence of rhlA, rhlB and the whole noncoding sequence between rhlA and rhlB. Preserving this noncoding sequence here is to ensure the accurate expression of rhamnosyltransferase I. Besides, a part of downstream noncoding sequence of rhlB is also included for composition with rhlRI, see our part [https://parts.igem.org/Part:BBa_K1331005 BBa_K1331005].
  
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===Usage and Biology===
 
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This part encodes both the subunit A and B of rhamnosyltransferase I, which is the essential protein for rhamnolipid synthesis.
 
 
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Latest revision as of 09:12, 19 October 2014

Modified rhlAB coding sequence from Pseudomonas aeruginosa SQ6


This is a composite part made of BBa_K1331001 and BBa_K1331004.

Usage and Biology

This part encodes both the subunit A and B, and together make the functional rhamnosyltransferase I, the essential enzyme for rhamnolipid synthesis. This sequence includes both the coding sequence of rhlA, rhlB and the whole noncoding sequence between rhlA and rhlB. Preserving this noncoding sequence here is to ensure the accurate expression of rhamnosyltransferase I. Besides, a part of downstream noncoding sequence of rhlB is also included for composition with rhlRI, see our part BBa_K1331005.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 69
    Illegal BamHI site found at 629
    Illegal XhoI site found at 805
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 992
    Illegal NgoMIV site found at 1713
    Illegal NgoMIV site found at 1826
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 294
    Illegal BsaI site found at 1342
    Illegal BsaI.rc site found at 478