Difference between revisions of "Part:BBa K1526000"
Line 2: | Line 2: | ||
<partinfo>BBa_K1526000 short</partinfo> | <partinfo>BBa_K1526000 short</partinfo> | ||
− | The phytochelatin synthase | + | The phytochelatin synthase gene from ''Schizosaccharomyces pombe''. |
Line 9: | Line 9: | ||
<b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group to synthesise phytochelatins.<br> | <b>Function:</b> The phytochelatin synthase in ''S. pombe'' uses glutathione with a blocked thiol group to synthesise phytochelatins.<br> | ||
<b>Aim:</b> Over-expression of this gene, together with Gsh1*, in <i>E.coli</i> has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.<br> | <b>Aim:</b> Over-expression of this gene, together with Gsh1*, in <i>E.coli</i> has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.<br> | ||
− | <b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol | + | <b>Literature</b><ol><li>http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/</li></ol> |
<!-- --> | <!-- --> |
Latest revision as of 15:41, 18 October 2014
SpPCS: Phytochelatin synthase from Schizosaccharomyces pombe
The phytochelatin synthase gene from Schizosaccharomyces pombe.
Gene: spPCS
Organism: Schizosaccharomyces pombe
Function: The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group to synthesise phytochelatins.
Aim: Over-expression of this gene, together with Gsh1*, in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation. We want to use this gene to make our bacteria more efficient in taking up cadmium.
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 339
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]