Difference between revisions of "Part:BBa K1391112:Design"

 
 
(One intermediate revision by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1391112 short</partinfo>
 
<partinfo>BBa_K1391112 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
None
+
This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 20:36, 1 November 2014

pEXPR_Tret:Cofilin (Inactivated - pseudophosphorylated)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 954
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NotI site found at 926
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 954
    Illegal BglII site found at 12
    Illegal XhoI site found at 921
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 954
    Illegal XbaI site found at 915
    Illegal SpeI site found at 249
    Illegal PstI site found at 949
    Illegal PstI site found at 2797
    Illegal NgoMIV site found at 1907
    Illegal NgoMIV site found at 3248
    Illegal NgoMIV site found at 3531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 1233
    Illegal BsaI.rc site found at 5056
    Illegal SapI site found at 1073
    Illegal SapI site found at 3976
    Illegal SapI.rc site found at 3097
    Illegal SapI.rc site found at 3307


Design Notes

This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

Human

References