Difference between revisions of "Part:BBa K1365020:Design"
(→References) |
(→References) |
||
Line 20: | Line 20: | ||
===References=== | ===References=== | ||
− | Overkamp, W. et al (2013) Benchmarking Various Green Fluorescent Protein Variants in ''Bacillus subtilis'', ''Streptococcus pneumoniae'', and ''Lactococcus lactis'' for Live Cell Imaging. Appl. Env. Microbiol. 79: 6481-6490. | + | Overkamp, W. et al (2013) Benchmarking Various Green Fluorescent Protein Variants in ''Bacillus subtilis'', ''Streptococcus pneumoniae'', and ''Lactococcus lactis'' for Live Cell Imaging. ''Appl. Env. Microbiol.'' 79: 6481-6490. |
Latest revision as of 02:52, 18 October 2014
sfGFP(Bs)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13
Design Notes
Used primers:
forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGTCAAAAGGAGAAGAGCT-3'
reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACTTATAAAGCTCAT-3'
to PCR sfGFP(Bs) from the plasmid and add the BioBrick prefix and suffix.
Source
PCR on plasmid MG_sfgfp(Bs), used in the study 'Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging', see references.
Plasmid kindly provided by the Molecular Genetics group of the Rijksuniversiteit Groningen.
References
Overkamp, W. et al (2013) Benchmarking Various Green Fluorescent Protein Variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for Live Cell Imaging. Appl. Env. Microbiol. 79: 6481-6490.