Difference between revisions of "Part:BBa K1391105:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts. | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:15, 30 October 2014
pEXPR_hEF1a:CD79B-TCS-Gal4VP16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was created using a single pot LR reaction. The promoter is flanked by B4 and P1 sites and the gene is flanked by P1 and B2 sites. Either of these can be extracted using a BP reaction. This part adheres to RFC 65 for recombination based cloning of mammalian parts.
Source
Human genome, viral genome, yeast genome