Difference between revisions of "Part:BBa K1365020"
 
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[[File:sfgfpbs.png]]
 
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===Sequence and features===
 
===Sequence and features===

Latest revision as of 02:52, 18 October 2014

sfGFP(Bs)

Usage and Biology

This gene codes for the sfGFP(Bs) protein, an optimized fluorescent reporter. This sfGFP was originally optimized for Bacillus subtilis. When it was tested in different species it showed high fluorescence in Lactococcus lactis. The sfGFP(Bs) has a very high performance in L. lactis compared to other GFP proteins, and also works really well in other bacteria like B. subtilis, Escherischia coli and Streptococcus pneumoniae.

Sfgfpbs.png


Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 13


References

Overkamp, W. et al. (2013) Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging. Appl. Environ. Microbiol. 79: 6481-6490