Difference between revisions of "Part:BBa K1431824:Experience"

Latest revision as of 13:06, 23 October 2014

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

At 1:50 (16th,Oct.)

At 3:00 (16th,Oct.)

At 6:00 (16th,Oct.)

At 7:40 (16th,Oct.)

At 8:00 (16th,Oct.)

At 10:00 (16th,Oct.)

At 11:20 (16th,Oct.)

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

At 02:45 (17th,Oct.)

At 04:55 (17th,Oct.)

At 10:40 (17th,Oct.)

Conclusion

With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.

User Reviews

UNIQ459f21a2de9656e3-partinfo-00000000-QINU UNIQ459f21a2de9656e3-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
-__NOTOC__
-This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
-how you used this part and how it worked out.
 ===Applications of BBa_K1431824===
@@ Line 58: / Line 55: @@
-At 22:40 (17th,Oct.)
+At 10:40 (17th,Oct.)
 <center>https://static.igem.org/mediawiki/parts/9/95/SUSTC_31_916_L-4.jpg </center>