Difference between revisions of "Part:BBa K1400002"
 
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This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.
 
This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.
  
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<html><img src="https://static.igem.org/mediawiki/2014/1/1c/Pgal4sites.png" /></html>
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<html><img style="width:55%;" src="https://static.igem.org/mediawiki/2014/1/1c/Pgal4sites.png" /></html>
  
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Figure 1: Characterization of pGAL via dual drug induction. pGAL has no pressing sites and 4 activating gal4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence.  
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Figure 1: Characterization of pGAL via dual drug induction. pGAL has no repressing sites and 4 activating GAL4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence. The fusion protein, GEV (GAL4 binding domain-human estrogen receptor-VP16 activator domain), is the activator and is expressed with the weak constitutive promoter, pMRP7.
  
 
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Latest revision as of 02:45, 18 October 2014

PGAL Modified native pGAL

This is the native pGAL1 promoter in S. cerevisiae with its Mig1 sites removed. This is a single input promoter that has four upstream activating sequences (UAS), which are GAL4 binding elements. The Mig1 sites are binding sites for proteins responsible for transcriptional repression of downstream genes to this and other promoters in S. cerevisiae in the presence of glucose. The removal of the Mig1 sites allows transcriptional activation of the promoter in the presence of glucose in the cellular growth medium.

Figure 1: Characterization of pGAL via dual drug induction. pGAL has no repressing sites and 4 activating GAL4 sites, so increasing estradiol increases activation while aTc has no effect beyond auto-fluorescence. The fusion protein, GEV (GAL4 binding domain-human estrogen receptor-VP16 activator domain), is the activator and is expressed with the weak constitutive promoter, pMRP7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 76
  • 1000
    COMPATIBLE WITH RFC[1000]