Difference between revisions of "Part:BBa K1502001:Design"

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===Source===
 
===Source===
  
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This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene.  
+
This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene (#49809).
  
 
===References===
 
===References===
  
 
(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.
 
(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.

Latest revision as of 23:47, 18 October 2014


accA portion of Acyl-CoA Ligase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 517
    Illegal SapI.rc site found at 714


Design Notes

The PCR process included X and P overhangs in order to include into the pSB1C3 plasmid.


Source

This part was isolated through PCR from the pACM4-ACA plasmid ordered from AddGene (#49809).

References

(2010) Microbial production of fatty-acid-derived fuels and chemicals from plant biomass. Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre and Jay D. Keasling. Nature. 275: 28593-28598.