Difference between revisions of "Part:BBa K1453408:Design"
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===Source=== | ===Source=== | ||
− | E.coli, BBa_K1453406, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. | + | E.coli, BBa_K1453006, BBa_K1453406, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. |
===References=== | ===References=== |
Latest revision as of 21:18, 17 October 2014
pykF-TAL1-His Tag
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1312
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1312
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1027
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1312
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1312
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1933
Illegal BsaI.rc site found at 2545
Design Notes
This part can recognize a special sequence, T-TCGATATCAAGC-T. This part contains the pyruvate kinase (EC:2.7.1.40).
Source
E.coli, BBa_K1453006, BBa_K1453406, BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.