Difference between revisions of "Part:BBa K1498000:Experience"
 
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===Applications of BBa_K1498000===
 
===Applications of BBa_K1498000===
  
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Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 36.5 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity.  
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Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.
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https://static.igem.org/mediawiki/2014/d/d5/Kerat2_Sheffield2014.png
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<br>
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Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
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Lane 3: Secreted keratinase
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In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).
 
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As a protease gelatin degredation should also be observed.
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 03:21, 18 October 2014

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Applications of BBa_K1498000

Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity. 

Kerat2_Sheffield2014.png


Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
Lane 3: Secreted keratinase


User Reviews

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