Difference between revisions of "Part:BBa K1498000:Experience"

Latest revision as of 03:21, 18 October 2014

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Applications of BBa_K1498000

Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.

Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
Lane 3: Secreted keratinase

User Reviews

UNIQb16958c1a3e2f8b5-partinfo-00000000-QINU UNIQb16958c1a3e2f8b5-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K1498000===
-In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).
+Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.
+ https://static.igem.org/mediawiki/2014/d/d5/Kerat2_Sheffield2014.png
+<br>
+Lane 1: EZ run protein ladder (Orange marker 72kDa, then 55, 40 and 33 kDa).
+<br>
+Lane 3: Secreted keratinase
+<br>
-As a protease gelatin degredation should be observed.
 ===User Reviews===