Difference between revisions of "Part:BBa K1453005"
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<partinfo>BBa_K1453005 short</partinfo> | <partinfo>BBa_K1453005 short</partinfo> | ||
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This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test. | This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test. | ||
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https://static.igem.org/mediawiki/2014/4/41/Membrane_TAL1.png | https://static.igem.org/mediawiki/2014/4/41/Membrane_TAL1.png | ||
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+ | '''Figure 1 ssDsbA-mRFP-Lgt-TAL1-His Tag''' | ||
The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. | The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. |
Latest revision as of 18:44, 17 October 2014
ssDsbA-mRFP-Lgt-TAL1-His Tag
This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test.
Structure
Figure 1 ssDsbA-mRFP-Lgt-TAL1-His Tag
The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 612
Illegal AgeI site found at 724
Illegal AgeI site found at 1537 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1158
Illegal BsaI.rc site found at 2176
Illegal BsaI.rc site found at 2788