Difference between revisions of "Part:BBa K1509002:Design"

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===Design Notes===
 
===Design Notes===
 
It was attained from ''Bacillus sp''. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:
 
It was attained from ''Bacillus sp''. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:
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P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’
 
P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’
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P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’
 
P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’
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And the PCR runs under the settings as follows:
 
And the PCR runs under the settings as follows:
  
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Temperature：     96℃    94℃    50℃    72℃    72℃
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[[File:Flocculation_PCR.png]]
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Time：              15min  40sec    60sec  4min    7min
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Number of cycle：    1                30              1
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Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
 
Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.
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==='''Sourse'''===
 
==='''Sourse'''===
 
It’s origin is one strain named as ''Bacillus sp''. F2, whose flocculent ability can reach 84%.
 
It’s origin is one strain named as ''Bacillus sp''. F2, whose flocculent ability can reach 84%.
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==='''References'''===
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1.  Fang Ma, Junliang Liu, Shugeng  Li, Jixian Yang, Liqiu Zhang, Bo Wu, Yanbin Zhu.''Development of complex microbial flocculant''.  China water and wastewater, 2003, 19(4):1-5.
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2.  Yuguang Chang, Fang Ma, Jingbo  Guo, Nanqi Ren. <em>Flocculent genomic clone  and flocculating mechanism analysis</em>. Environmental Science, 2007,  28(12):2849-2855.
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3.  Soares, E.V., <em>Flocculation in Saccharomyces cerevisiae: a review.</em> Journal of  Applied Microbiology, 2011. <strong>110</strong>(1): p. 1-18.

Latest revision as of 01:22, 18 October 2014

Design Notes

It was attained from Bacillus sp. F2 by using PCR with the total genomic DNA of F2 as template. The two synthesized oligonucleotide primers were shown as below:

P1: 5’-AGTCTAGATGTAAGCTCTCTTCCGG-3’

P2:5’-CTCTCGAGCAATAAGGACGCAATG-3’

And the PCR runs under the settings as follows:

Flocculation PCR.png

Then it was spliced with the DNA sequence for peptide CDS 7 with a synthetic constitutive promoters CP25.

Sourse

It’s origin is one strain named as Bacillus sp. F2, whose flocculent ability can reach 84%.

References

1. Fang Ma, Junliang Liu, Shugeng Li, Jixian Yang, Liqiu Zhang, Bo Wu, Yanbin Zhu.Development of complex microbial flocculant. China water and wastewater, 2003, 19(4):1-5.

2. Yuguang Chang, Fang Ma, Jingbo Guo, Nanqi Ren. Flocculent genomic clone and flocculating mechanism analysis. Environmental Science, 2007, 28(12):2849-2855.

3. Soares, E.V., Flocculation in Saccharomyces cerevisiae: a review. Journal of Applied Microbiology, 2011. 110(1): p. 1-18.