Difference between revisions of "Part:BBa K1351029"

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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K1351028 short</partinfo>
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<partinfo>BBa_K1351029 short</partinfo>
  
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This part was generated in a modified version of RFC10, and has the following prefix and suffix:  
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This part was generated in RFC10, and has the following prefix and suffix:  
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              Prefix                                                                  Suffix
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5' - GAATTC GCGGCCGC T TCTAGA G agaggaggata T ACTAGT A GCGGCCG CTGCAG - 3'
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      EcoRI    NotI      XbaI                                        SpeI    NotI  PstI
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This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
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This library was generated utilizing the [https://salis.psu.edu/software/ Salis lab RBS calculator] to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:
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{| class=wikitable
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|-
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!Number
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!BBa_K1310 ..
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!Sequence
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|-
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|1
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|28
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|aaggagggata
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|-
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|<b>2</b>
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|<b>29</b>
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|<b>agaggaggata</b>
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|-
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|3
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|30
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|aaggagagata
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|-
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|4
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|31
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|aggagaggata
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|-
 +
|5
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|32
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|aagaggagata
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|-
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|6
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|33
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|agaaagggata
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|-
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|7
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|34
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|aagaagagata
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|}
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The coverage of RBS strength as calculated by the [https://salis.psu.edu/software/ RBS calculator] is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.
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[[File:LMU_14_RBSlibrary.png|thumb|center|400px|Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the [https://salis.psu.edu/software/ RBS calculator]]]
  
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{||prefix with EcoRI, NotI, XbaI, SD and NgoMIV:|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>TG<span style="color:orange">GCCGGC</span>|-|suffix with AgeI, SpeI, NotI and PstI:|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>|}Sites of restriction enzymes generating compatible overhangs have the same color:
 
  
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<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Stop codons are underlined.
 
  
  

Latest revision as of 06:33, 1 November 2014

LMU Bacillus RBS collection 2

This part was generated in RFC10, and has the following prefix and suffix: 

             Prefix                                                                   Suffix
5' - GAATTC GCGGCCGC T TCTAGA G agaggaggata T ACTAGT A GCGGCCG CTGCAG - 3'
     EcoRI    NotI      XbaI                                         SpeI     NotI   PstI


This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

This library was generated utilizing the Salis lab RBS calculator to find RBS that cover a high space of translation intitiation rate in 'B. subtilis'. It includes seven RBS with the following sequences:

Number BBa_K1310 .. Sequence
1 28 aaggagggata
2 29 agaggaggata
3 30 aaggagagata
4 31 aggagaggata
5 32 aagaggagata
6 33 agaaagggata
7 34 aagaagagata

The coverage of RBS strength as calculated by the RBS calculator is depicted in the Figure 1 shown below. It shows an evenly distributed strength spanning approxiamately three orders of magnitude.

Fig.1: Coverage of the translation initiation rate of the RBS library as calculated by the RBS calculator



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]