Difference between revisions of "Part:BBa K1462110"
 
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This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
 
This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
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[[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
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[[File:GEG2.jpg|200px|left|frame|Figure 1. <b>Enzymes located in cytoplasm</b> The fluorescence indicates that Erg10 is expressed in the cytoplasm]]
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1462110 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1462110 SequenceAndFeatures</partinfo>
  

Latest revision as of 12:10, 17 October 2014

Gal1+Erg10+GFP+ADH1


This part is constructed as a control group to identify our enzymes have been directed to the mitochondria.The GFP is fused at the C-terminus of Erg10 to report where the enzyme located.Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Figure 1. Enzymes located in cytoplasm The fluorescence indicates that Erg10 is expressed in the cytoplasm



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1252
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2402