Difference between revisions of "Part:BBa K1316017"
 
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A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.
 
A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.
  
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<h3> Important note </h3>
 
<h3> Important note </h3>
 
There was, unfortunately, a mistake in the design of the reverse primer, which caused the STOP codon of MtrB not to be placed right after the His Tag (where is shoul be) but some base pairs behind.  
 
There was, unfortunately, a mistake in the design of the reverse primer, which caused the STOP codon of MtrB not to be placed right after the His Tag (where is shoul be) but some base pairs behind.  
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<h2> Characterisation </h2>
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Due to the mistake in the sequence, no further characterisation was carried out for this construct.
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For more interest on characterisation of the mtrCAB genes check our BioBrick
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<a href="https://parts.igem.org/Part:BBa_K1316012"> BBa_K1316012  </a> or our <a href="http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/EET/characterisation#top"> Electron Transport characterisation wiki page </a> !
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Latest revision as of 09:48, 17 October 2014

T7 lacO + mtrCAB with His tag

Combination of T7 promoter with the lac operator. For the promoter to be active both T7 RNA polymerase and the lac operon inducer (lactose or an analogue such as IPTG) must be present. This double regulatory system reduces the promoter leakage MtrCAB is an electron transport complex of the bacteria Shewanella oneidensis

A His tag was placed at the C-terminus of the MtrB protein in order to easily be able to detect and purify this protein.

Important note

There was, unfortunately, a mistake in the design of the reverse primer, which caused the STOP codon of MtrB not to be placed right after the His Tag (where is shoul be) but some base pairs behind.

Characterisation

Due to the mistake in the sequence, no further characterisation was carried out for this construct.


For more interest on characterisation of the mtrCAB genes check our BioBrick BBa_K1316012 or our Electron Transport characterisation wiki page !



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 359