Difference between revisions of "Part:BBa K1373001:Design"
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R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc | R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc | ||
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| + | ===Usage and Biology=== | ||
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Latest revision as of 07:56, 17 October 2014
nadE with strong promter and strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
cloning via omega-PCR
Primers
F-nadE:ctagctactagagaaagaggagaaaATGACATTGCAACAACAAATAATAAAG
R-nadE:GATGATTTCTGGAAAAAGTAAtactagtagcggccgctgcagtc
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655
References
1.Yong, X.-Y. et al. Enhancement of bioelectricity generation by cofactor manipulation in microbial fuel cell. Biosensorsand Bioelectronics 56, 19-25, doi:http://dx.doi.org/10.1016/j.bios.2013.12.058 (2014).
2.Chen L, Wang F, Wang X, Liu YG. (2013) Robust one-tube Ω-PCR strategy accelerates precise sequence modification of plasmids for functional genomics. Plant Cell Physiology