Difference between revisions of "Part:BBa K1480003"

(→‎Results)
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Here are the links for the other parts used.
 
Here are the links for the other parts used.
  
−
https://parts.igem.org/Part:BBa_K1140003 <br>
+
https://parts.igem.org/Part:BBa_K1140006 <br>
 
https://parts.igem.org/Part:BBa_K1480003 <br>
 
https://parts.igem.org/Part:BBa_K1480003 <br>
 
https://parts.igem.org/Part:BBa_K145201 <br>
 
https://parts.igem.org/Part:BBa_K145201 <br>

Latest revision as of 20:33, 4 August 2015

TetR generator (Promoter J23109)

This is a TetR protein generator regulated by the constitutive promoter J23109.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

https://parts.igem.org/Part:BBa_K1140006
https://parts.igem.org/Part:BBa_K1480003
https://parts.igem.org/Part:BBa_K145201
https://parts.igem.org/Part:BBa_K1480004
https://parts.igem.org/Part:BBa_K1480005


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]