Difference between revisions of "Part:BBa K1323004"
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<partinfo>BBa_K1323004 short</partinfo> | <partinfo>BBa_K1323004 short</partinfo> | ||
| − | Hfq is a protein which enhances sRNA complementation to its mRNA target - | + | Hfq is a protein which enhances sRNA complementation to its mRNA target - it does so by binding AU-rich and poly-A-rich sequences. sRNAs which associate with Hfq have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) ''E. coli'' contain the Hfq gene in their chromosomes - other species, including ''Staphylococcus sp''., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in ''S. epidermidis'' using JCat software. |
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| + | This part was DNA synthesized by Bio Basic. | ||
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==References== | ==References== | ||
| − | Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocol 8(9), 1694-1707. | + | Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in ''Escherichia coli''. ''Nature protocol'' '''8'''(9), 1694-1707. |
Latest revision as of 02:22, 22 October 2014
Hfq: Expression cassette under a xylose inducible promoter
Hfq is a protein which enhances sRNA complementation to its mRNA target - it does so by binding AU-rich and poly-A-rich sequences. sRNAs which associate with Hfq have greater target binding rate, target mRNA degradation, and half-life (Yoo et al., 2013). Species which are closely related to (and including) E. coli contain the Hfq gene in their chromosomes - other species, including Staphylococcus sp., will require this expression cassette if heightened sRNA activity is to be achieved. Illegal sites were removed and the sequence was codon optimized for expression in S. epidermidis using JCat software.
This part was DNA synthesized by Bio Basic.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1476
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Yoo, S, M., Na, D., Lee, S. Y. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature protocol 8(9), 1694-1707.