Difference between revisions of "Part:BBa K1480003"

Latest revision as of 20:33, 4 August 2015

TetR generator (Promoter J23109)

This is a TetR protein generator regulated by the constitutive promoter J23109.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

https://parts.igem.org/Part:BBa_K1140006
https://parts.igem.org/Part:BBa_K1480003
https://parts.igem.org/Part:BBa_K145201
https://parts.igem.org/Part:BBa_K1480004
https://parts.igem.org/Part:BBa_K1480005

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 The protein has a LVA tag for degradation.
+==Usage and Biology==
+It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.
 ==Results==
@@ Line 11: / Line 15: @@
 Here are the links for the other parts used.
- [[Link:https://parts.igem.org/Part:BBa_K1140003|K1140003]]
- [[Link:https://parts.igem.org/Part:BBa_K1480003|K1480003]]
+https://parts.igem.org/Part:BBa_K1140006 <br>
- [[Link:https://parts.igem.org/Part:BBa_K145201|k145201]]
+https://parts.igem.org/Part:BBa_K1480003 <br>
- [[Link:https://parts.igem.org/Part:BBa_K1480004|K1480004]]
+https://parts.igem.org/Part:BBa_K145201 <br>
- [[Link:https://parts.igem.org/Part:BBa_K1480005|K1480005]]
+https://parts.igem.org/Part:BBa_K1480004 <br>
+https://parts.igem.org/Part:BBa_K1480005