Difference between revisions of "Part:BBa K1398005"

Latest revision as of 19:19, 2 November 2014

NemR Upstream Intergenic Region

This sequence is found upstream of several NemR regulated genes. It was created for use as a TNT-detection mechanism. It contains the bases from 220+ to the start codon, which include a promoter and RBS. This region should allow regulation of gene preoducts through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.

An iLOV reporter was added to this construct to characterize the response to concentrations of TNT. This part can be found here:

https://parts.igem.org/Part:BBa_K1398004

The full article detailing the characterisation method can be found here:

http://2014.igem.org/Team:Exeter/Detection

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 It contains the bases from 220+ to the start codon, which include a promoter and RBS.
 This region should allow regulation of gene preoducts through the detection of TNT. If it is not present NemR will bind to a specified sequence of bases and inhibit transcription. If TNT is not present NemR will not bind and transcription will complete.
+An iLOV reporter was added to this construct to characterize the response to concentrations of TNT. This part can be found here:
+https://parts.igem.org/Part:BBa_K1398004
+The full article detailing the characterisation method can be found here:
+http://2014.igem.org/Team:Exeter/Detection