Difference between revisions of "Part:BBa K1442040:Design"

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[3] Auslander, S., Auslander, D., Muller, M., Wieland, M., Fussenegger, M., 2012, Programmable single-cell mammalian biocomputers, Nature, 487:123–127, http://www.nature.com/nature/journal/v487/n7405/full/nature11149.html?WT.ec_id=NATURE-20120705
 
[3] Auslander, S., Auslander, D., Muller, M., Wieland, M., Fussenegger, M., 2012, Programmable single-cell mammalian biocomputers, Nature, 487:123–127, http://www.nature.com/nature/journal/v487/n7405/full/nature11149.html?WT.ec_id=NATURE-20120705
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[4] Peabody, D.S., 1990, Translational Repression by Bacteriophage MS2 Coat Protein Expressed from a Plasmid: A SYSTEM FOR GENETIC ANALYSIS OF A PROTEIN-RNA INTERACTION*, The Journal of Biological Chemistry, 265:10, 5684-5689: http://www.ncbi.nlm.nih.gov/pubmed/2108146

Latest revision as of 19:28, 16 October 2014

MS2 bacteriophage coat protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 255
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 255
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 255
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 255
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 255
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 292


Design Notes

The MS2 coat protein was codon optimised before it was ordered within the replicon by IDT

Source

The MS2 box/protein was synthesised within our replicon by IDT.

References

[1] Peabody, D.S., 1993, The RNA binding site of bacteriophage MS2 coat protein., EMBO J. Feb 1993; 12(2): 595–600, http://www.ncbi.nlm.nih.gov/pmc/articles/PMC413242/

[2] Isaacs, F.J., Dwyer, D.J., Collins, J.J., 2006, RNA synthetic biology, Nature Biotechnology, 24: 545-554, http://www.nature.com/nbt/journal/v24/n5/full/nbt1208.html

[3] Auslander, S., Auslander, D., Muller, M., Wieland, M., Fussenegger, M., 2012, Programmable single-cell mammalian biocomputers, Nature, 487:123–127, http://www.nature.com/nature/journal/v487/n7405/full/nature11149.html?WT.ec_id=NATURE-20120705

[4] Peabody, D.S., 1990, Translational Repression by Bacteriophage MS2 Coat Protein Expressed from a Plasmid: A SYSTEM FOR GENETIC ANALYSIS OF A PROTEIN-RNA INTERACTION*, The Journal of Biological Chemistry, 265:10, 5684-5689: http://www.ncbi.nlm.nih.gov/pubmed/2108146