Difference between revisions of "Part:BBa K1431413:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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After we use design the gRNA of HBV-2(BBa_K1431403), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .
+
After we use design the gRNA of HBV-2([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1431403 BBa_K1431403]), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .
  
 
But there are two problem:
 
But there are two problem:
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Second,the whole HBV genome have a potential dangers in the lab.
 
Second,the whole HBV genome have a potential dangers in the lab.
  
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So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of binding swquence to the change of reporter.
+
So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of binding sequence to the change of reporter.
 
The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.
 
The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.
  
 
===Source===
 
===Source===
  
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we will complete this part later
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AB010289-AB078031 in HBV_aligned
  
 
===References===
 
===References===

Latest revision as of 09:47, 17 October 2014

target sequence2 for HBV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

After we use design the gRNA of HBV-2(BBa_K1431403), we need the corresponding binding sequence DNA to test the efficiency of gRNA to lead a double strands break .

But there are two problem:

First,the DSB of binding sequence is hard to observe in the cell;

Second,the whole HBV genome have a potential dangers in the lab.

So we use the alternative solution.We insert the gRNA binding sequence between the promoter and reporter, transform the expression of DSB of binding sequence to the change of reporter. The whole target-sequence have a gRNA binding sequence in the middle and have more original sequence in two sides to return the natural state of HBV genome.

Source

AB010289-AB078031 in HBV_aligned

References