Difference between revisions of "Part:BBa K1326004"

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Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway.
 
Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway.
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Magnesium chelatase subunit I (ChlI1) and Magnesium chelatase subunit D (ChlD) form an ATP-dependent complex (increased yield is seen if ChlI2 subunit is also present). This complex acts on protoporphyrin IX, bound to the ChlH protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX.
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Magnesium chelatase subunit I ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080000 ChlI1]) and Magnesium chelatase subunit D ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080002 ChlD]) form an ATP-dependent complex (increased yield is seen if [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080011 ChlI2] subunit is also present). This complex acts on protoporphyrin IX, bound to the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080001 ChlH] protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX.
 
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===Usage and Biology===
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Has since been added to: [https://parts.igem.org/Part:BBa_K1326008 BBa_K1326008]
 
Has since been added to: [https://parts.igem.org/Part:BBa_K1326008 BBa_K1326008]
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This composite part was used to add further parts on to to form a functional operon. The two genes present have been found to be functional, converting Protoporphyrin IX into Mg-protoporphyrin IX. These results can bee seen below in Functional Parameters.
 
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===Usage and Biology===
 
  
 
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[[File:Fluorescence Spectra of ChlI1 + ChlD at 420nm Excitation.png]]
 
[[File:Fluorescence Spectra of ChlI1 + ChlD at 420nm Excitation.png]]
 
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'''''Figure 1:''''' /ChlI1 + ChlD/ composite part has converted Mg-Protoporphyrn IX into Protoporphyrin IX.
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'''''Figure 1:''''' Fluorescence spectra at 420 nm excitation of induced ChlI1+ChlD extract following incubation in Figure 1. Peak observed at 595nm is consistent with Mg-Protoporphyrin IX production, demonstrating significant activity of the submitted BioBrick part.
 
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[[File:Fluorescence Intensity of Mg-Protoporphyrin Formation.png]]
 
[[File:Fluorescence Intensity of Mg-Protoporphyrin Formation.png]]

Latest revision as of 02:56, 23 October 2014

Plac + ChlI1 + ChlD

Composite part incorporating the genes adding a Mg ion to protoporphyrin IX, as a part of the chlorophyll biosynthetic pathway. Magnesium chelatase subunit I (ChlI1) and Magnesium chelatase subunit D (ChlD) form an ATP-dependent complex (increased yield is seen if ChlI2 subunit is also present). This complex acts on protoporphyrin IX, bound to the ChlH protein, and inserts a magnesium ion which produces Mg-protoporphyrin IX.

Usage and Biology

Has since been added to: BBa_K1326008
This composite part was used to add further parts on to to form a functional operon. The two genes present have been found to be functional, converting Protoporphyrin IX into Mg-protoporphyrin IX. These results can bee seen below in Functional Parameters.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 439
    Illegal BglII site found at 1095
    Illegal BglII site found at 1290
    Illegal BglII site found at 3371
    Illegal BamHI site found at 487
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1946
    Illegal NgoMIV site found at 2765
    Illegal NgoMIV site found at 3307
  • 1000
    COMPATIBLE WITH RFC[1000]



Functional Parameters


Fluorescence Spectra of ChlI1 + ChlD at 420nm Excitation.png
Figure 1: Fluorescence spectra at 420 nm excitation of induced ChlI1+ChlD extract following incubation in Figure 1. Peak observed at 595nm is consistent with Mg-Protoporphyrin IX production, demonstrating significant activity of the submitted BioBrick part.
Fluorescence Intensity of Mg-Protoporphyrin Formation.png
Figure 2: Increasing fluorescence intensity over time through function of /ChlI1 + ChlD/ composite part..