Difference between revisions of "Part:BBa K1453300"

 
 
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<partinfo>BBa_K1453300 short</partinfo>
 
<partinfo>BBa_K1453300 short</partinfo>
  
Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, so the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTGGTCATGAGA(12bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.
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[[File:fl3tal.png|border|600px]]
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Figure The structure of FL-TAL1
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This is the TALE protein with a flexible linker 3 before it. Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTTGGTCATGAGAT(14bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.
  
 
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Latest revision as of 00:01, 17 October 2014

FL3-TALE

Fl3tal.png

Figure The structure of FL-TAL1

This is the TALE protein with a flexible linker 3 before it. Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTTGGTCATGAGAT(14bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 526
    Illegal BsaI.rc site found at 1138