Difference between revisions of "Part:BBa K1453300"
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<partinfo>BBa_K1453300 short</partinfo> | <partinfo>BBa_K1453300 short</partinfo> | ||
− | Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, | + | [[File:fl3tal.png|border|600px]] |
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+ | Figure The structure of FL-TAL1 | ||
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+ | This is the TALE protein with a flexible linker 3 before it. Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTTGGTCATGAGAT(14bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 00:01, 17 October 2014
FL3-TALE
Figure The structure of FL-TAL1
This is the TALE protein with a flexible linker 3 before it. Since we cannot connect TALE by Golden Gate method designed by 2012 Freiburg, the sequence was synthesized by Genwize company. This TALE can recognize the DNA sequence TTTGGTCATGAGAT(14bp). Moreover, we use this part with our part BBa_K14530000 to make our composite part BBa_K1453305.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 526
Illegal BsaI.rc site found at 1138