Difference between revisions of "Part:BBa K1172401:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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<partinfo>BBa_K1172401 AddReview 1</partinfo> | <partinfo>BBa_K1172401 AddReview 1</partinfo> | ||
| − | <I>TUDelft 2014</I> | + | <I>TUDelft-Leiden 2014</I> |
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
We could not detect the coding sequence of mtrCAB by restriction analysis of part BBa_K1172401 or by sequencing the part BBa_K1172403 (mtrCAB with medium Promoter and medium RBS), which should contain the mtrCAB coding region of BBa_K1172401 according to Bielefeld 2013 iGEM team. | We could not detect the coding sequence of mtrCAB by restriction analysis of part BBa_K1172401 or by sequencing the part BBa_K1172403 (mtrCAB with medium Promoter and medium RBS), which should contain the mtrCAB coding region of BBa_K1172401 according to Bielefeld 2013 iGEM team. | ||
| + | TUDelft-Leiden 2014 iGEM team has created part BBa_K1316012 which contains the proper mtrCAB coding sequence on it and, therefore represents an improvement of this part. | ||
<html> | <html> | ||
| − | + | <p> | |
| − | <ul>Sequencing of BBa_K1172403: | + | <ul> |
| − | < | + | <li>Sequencing of BBa_K1172403: |
| − | < | + | </p> |
| + | <p> <a href="https://static.igem.org/mediawiki/2014/f/f3/TUDelft_2014_sequencing_Bielef_VF2.pdf">Using the iGEM standard forward primer (VF2)</a> | ||
| + | </p> | ||
| + | <p> <a href="https://static.igem.org/mediawiki/2014/0/00/TUDelft_2014_sequencing_Bielef.pdf">Using the iGEM standard reverse primer (VR)</a></p> | ||
| + | </li> | ||
| + | |||
| + | <li>Restriction Analysis of BBa_K1172401: | ||
| + | <a href="https://static.igem.org/mediawiki/2014/7/7e/TUDelft_2014_Bielefeld_restriction.png">Click here</a> to see the picture of the gel. The first lane is smart ladder, sizes: 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 800, 600, 400 and 200 bp. The second lane corresponds to 5ul of BioBrick K1172401 digested with EcoRI and XbaI. Therefore, the expected size of the band on lane 2 is 7.4 kb (BioBrick K1172401 + pSB1C3 Backbone). The actual size is, however, about 4.2 kb. The rest of the bands are not relevant. | ||
| + | </li> | ||
</ul> | </ul> | ||
| + | </p> | ||
| + | <br> | ||
| + | </ul> | ||
<!-- DON'T DELETE --><partinfo>BBa_K1172401 EndReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1172401 EndReviews</partinfo> | ||
Latest revision as of 17:33, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
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UNIQ6b3a778444cd844f-partinfo-00000000-QINU UNIQ6b3a778444cd844f-partinfo-00000001-QINU
•
TUDelft-Leiden 2014 |width='60%' valign='top'| We could not detect the coding sequence of mtrCAB by restriction analysis of part BBa_K1172401 or by sequencing the part BBa_K1172403 (mtrCAB with medium Promoter and medium RBS), which should contain the mtrCAB coding region of BBa_K1172401 according to Bielefeld 2013 iGEM team. TUDelft-Leiden 2014 iGEM team has created part BBa_K1316012 which contains the proper mtrCAB coding sequence on it and, therefore represents an improvement of this part.
- Sequencing of BBa_K1172403:
- Restriction Analysis of BBa_K1172401: Click here to see the picture of the gel. The first lane is smart ladder, sizes: 10000, 8000, 6000, 5000, 4000, 3000, 2500, 2000, 1500, 1000, 800, 600, 400 and 200 bp. The second lane corresponds to 5ul of BioBrick K1172401 digested with EcoRI and XbaI. Therefore, the expected size of the band on lane 2 is 7.4 kb (BioBrick K1172401 + pSB1C3 Backbone). The actual size is, however, about 4.2 kb. The rest of the bands are not relevant.
