Difference between revisions of "Part:BBa K1433003"
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<p>Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.</p> | <p>Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.</p> | ||
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| + | https://static.igem.org/mediawiki/parts/e/ee/ZJU_int-xis-bplr.gif | ||
<p><b><big>Composition</big></b><br /> | <p><b><big>Composition</big></b><br /> | ||
| − | <ol><li> Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.</li> | + | <ol><li> Promoter: [https://parts.igem.org/Part:BBa_J23110 BBa_J23110], a middle-ground Bacterial constitutive promoter.</li> |
<li>gp47: An excisionase in Mycobacterium phage Bxb1.</li> | <li>gp47: An excisionase in Mycobacterium phage Bxb1.</li> | ||
<li>tag: A distinct proteolysis tag on gp47 peptides.</li> | <li>tag: A distinct proteolysis tag on gp47 peptides.</li> | ||
| − | <li>terminator: BBa_B0015, a strong double terminator.</li></ol></p> | + | <li>terminator: [https://parts.igem.org/Part:BBa_B0015 BBa_B0015], a strong double terminator.</li></ol></p> |
| − | [[File: | + | [[File:ZJU-K1433003.jpg]] |
<p>This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.</p> | <p>This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.</p> | ||
Latest revision as of 12:28, 17 October 2014
RBS-gp47-tag-terminator
Bxb1 gp47 is an excisionase and Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase along can typically catalyzes site-specific recombination and mediate DNA inversion from an BP to LR state. Heterologous expression of Bxb1 integrase gp35 and excisionase gp47 could mediate DNA restore from an LR to BP state.
Composition
- Promoter: BBa_J23110, a middle-ground Bacterial constitutive promoter.
- gp47: An excisionase in Mycobacterium phage Bxb1.
- tag: A distinct proteolysis tag on gp47 peptides.
- terminator: BBa_B0015, a strong double terminator.
This part has component encodes a distinct proteolysis tag on gp47 peptides, for fine control over gp47 synthesis and degradation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 29
Illegal AgeI site found at 797 - 1000COMPATIBLE WITH RFC[1000]