Difference between revisions of "Part:BBa J58102"

 
 
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<partinfo>BBa_J58102 short</partinfo>
 
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Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated negatively (repressed) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors.  
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We use the promoter pOmpR <partinfo>R0082</partinfo> to interface with sensors that use a synthetic two-component signal transduction pathway based on an EnvZ domain (and a EnvZ- strain such as <partinfo>V1012</partinfo>). That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated).  
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The deep components are as follows:<partinfo>J58102 DeepComponents</partinfo>This composite part will be very useful to characterize many cellular biosensors.  
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'''By Valencia iGEM 2006'''
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''A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).''
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''1 Universidad Politecnica de Valencia, Spain''
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''2 Universitat de Valencia, Spain''
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''3 Ecole Polytechnique, France''
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:26, 29 October 2006

Promoter activated by OmpR-P with the reporter GFP

We use the promoter pOmpR BBa_R0082 to interface with sensors that use a synthetic two-component signal transduction pathway based on an EnvZ domain (and a EnvZ- strain such as BBa_V1012). That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated).

The deep components are as follows:
OmpR
R0082
B0034		GFP
E0040
B0010
B0012
This composite part will be very useful to characterize many cellular biosensors.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 778