Difference between revisions of "Part:BBa K1433002"
 
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<p>Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.
 
<p>Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.
 
</p>
 
</p>
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[[File:ZJU_int xis bplr reverse]]
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https://static.igem.org/mediawiki/parts/e/ee/ZJU_int-xis-bplr.gif
  
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<p><b><big>composition:</big></b><br />
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<p><b><big>Composition:</big></b><br />
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<ol>This part is composed of 2 elements.:
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<ol><li>Promoter (reverse): [https://parts.igem.org/Part:BBa_J23110 BBa_J23110], a middle-ground Bacterial constitutive promoter. </li>
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<li>Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter. </li>
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<li>attL and attR sites: Recognition sites for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.</li>
 
<li>attL and attR sites: Recognition sites for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.</li>
 
</ol></p>
 
</ol></p>
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<p>This part promotes the transcription and translation of the upstream genes. When it is treated with both of Bxb1 gp35 and gp47 at certain ratio, downstream gene will be expressed.</p>
 
<p>This part promotes the transcription and translation of the upstream genes. When it is treated with both of Bxb1 gp35 and gp47 at certain ratio, downstream gene will be expressed.</p>

Latest revision as of 12:24, 17 October 2014

attL-J23110-attR

Bxb1 gp35 is a serine integrase and Bxb1 gp47 is an excisionase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination and mediate DNA inversion from BP state to LR state. Expression of Bxb1 gp35 and gp47 at certain ratio could change DNA from LR state to BP state.

ZJU_int-xis-bplr.gif

Composition:

  1. Promoter (reverse): BBa_J23110, a middle-ground Bacterial constitutive promoter.
  2. attL and attR sites: Recognition sites for Bxb1 gp35, Mycobacterium Phage Bxb1 DNA integrase.


This part promotes the transcription and translation of the upstream genes. When it is treated with both of Bxb1 gp35 and gp47 at certain ratio, downstream gene will be expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 51
    Illegal NheI site found at 74
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 24
    Illegal BsaI.rc site found at 107