Difference between revisions of "Part:BBa K1460005:Experience"
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<h2>Cornell 2014 Results</h2> | <h2>Cornell 2014 Results</h2> | ||
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Cells successfully expressing CBP4 should be transporting more lead ions past the cell wall. This would lead to increased lead sensitivity. To test for lead sensitivity, <i>E.coli</i> BL21 and engineered BL21 with part BBa_K1460005 in the amp<sup>r</sup> plasmid pUC57 were grown for a 24 hour period in LB with 1 mM, Pb. | Cells successfully expressing CBP4 should be transporting more lead ions past the cell wall. This would lead to increased lead sensitivity. To test for lead sensitivity, <i>E.coli</i> BL21 and engineered BL21 with part BBa_K1460005 in the amp<sup>r</sup> plasmid pUC57 were grown for a 24 hour period in LB with 1 mM, Pb. | ||
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| − | What we see after 24 hours of growth is no significant difference in growth between the two strains. What we consistently observed as well, however, is that there is no inhibition of growth of BL21 at high concentrations of lead ( | + | What we see after 24 hours of growth is no significant difference in growth between the two strains (top chart). What we consistently observed as well, however, is that there is no inhibition of growth of BL21 at high concentrations of lead (bottom figure). Even if CBP4 is expressed and is actively transporting lead ions into cells, it is possible that the concentration of lead is still not high enough to be toxic to the organisms. We were, unfortunately, unable to test lead concentrations higher than those shown above because these working concentrations are approaching the maximum solubility of the lead nitrate that we were using for testing. |
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Latest revision as of 00:25, 16 October 2014
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Applications of BBa_K1460005
Cornell 2014 Results
Cells successfully expressing CBP4 should be transporting more lead ions past the cell wall. This would lead to increased lead sensitivity. To test for lead sensitivity, E.coli BL21 and engineered BL21 with part BBa_K1460005 in the ampr plasmid pUC57 were grown for a 24 hour period in LB with 1 mM, Pb.
What we see after 24 hours of growth is no significant difference in growth between the two strains (top chart). What we consistently observed as well, however, is that there is no inhibition of growth of BL21 at high concentrations of lead (bottom figure). Even if CBP4 is expressed and is actively transporting lead ions into cells, it is possible that the concentration of lead is still not high enough to be toxic to the organisms. We were, unfortunately, unable to test lead concentrations higher than those shown above because these working concentrations are approaching the maximum solubility of the lead nitrate that we were using for testing.